High Throughput Robotic Screening Facility
Our robotic facility combines two fully-automated fluorescent and brightfield confocal microscopes - Perkin Elmer's Opera & Cellomics' ArrayScan™ - for high resolution imaging with a Synergy HT plate reader that measures fluorescent targets and a KiNEDx robot that manipulates plates between machines.
The screening facility is capable of producing over 100,000 image sets per day, and allows us to quantify any physiological change in a target cell. Several parameters can be measured simultaneously within the same assay and/or cell, making this one of the fastest screening systems available.
Brand New Flow Cytometry Suite
We have our very own BD Fortessa X-20 flow cytometer on site at the CHEO RI. Boasting 4 lasers (violet 405nm, blue 488nm, yellow-green 561nm & red 633nm) and a high-throughput robotic sampler, we can perform robust detection of 18-colour stains.
We also have access to the Beckman Coulter CyAn ADP 9, a 3-laser machine with the ability to detect up to 9 fluorophores, and both manual and automatic loaders, a Beckman Coulter MoFlo Astrios capable of quickly and reliably sorting fluorescently labelled cells, and an AutoMACS Pro Magnetic cell separator from the core flow cytometry flow facility.
IVIS in vivo Imaging Facilities
Our Perkin Elmer in vivo imaging suite is used to track bioluminescent or fluorescent signals in a living animal. We often harness this high tech system to track the growth of tumours encoding firefly luciferase or analyse the bodywide distribution of oncolytic viruses engineered to express fluorescent tags.